- Nightshade is one of the ingredients in Skooma.
- The Black Sacrament requires nightshade.
- During the quest "Vampire Cure," five samples are needed to complete the cure.
- Growing in the Arcane University garden.
- On a table in the library of the Chorrol Mages Guild.
- Common around Skingrad, and southwest of Skingrad near a camp called Fat Rat Camp.
- Commonly sold at The Main Ingredient in the Imperial City.
- In the Shrine of Sithis in Deepscorn Hollow (5 samples).
- In Frostcrag Spire.
- Two in Fort Farragut in Lucien's room. One in the coffin and another on a plate.
Five samples are needed for the vampire cure potion.