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- Nightshade is one of the ingredients in Skooma.
- The Black Sacrament requires nightshade.
- During the quest Vampire Cure, five samples are needed to complete the cure.
- Growing in the Arcane University garden.
- On a table in the library of the Chorrol Mages Guild.
- Common around Skingrad, and southeast of Skingrad near a camp called Fat Rat Camp.
- Commonly sold at The Main Ingredient in the Imperial City.
- Several in Fort Farragut.
- In the Shrine of Sithis in Deepscorn Hollow (5 samples).
- In Frostcrag Spire.
Five samples are needed for the vampire cure potion.